Abstract The differences in nanohardness HIT between particular grains of non-oriented electrical steel at room and elevated (100, 200, 250 °C) temperature were observed using the nanoindentation technique. The elevated temperature indentation response was examined using an indentation Nano Test NT 600 system modified with tip and sample heating. Indentation hardness reveals descending tendency with increasing temperature in each of investigated grains with crystallographic orientations in the sheet plane – G1 {111}//ND, G2 {001}//ND, G3 {011} <001>. The indentation hardness showed some differences in values of indentation hardness between particular grain orientations. In the temperature range of 200-250 °C the load-depth (F-h) curves reveal the pop-in effect.